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There is increasing evidence that a wider range of lymphoid cell types other than CD4(+) T helper lymphocytes are infected with HIV-1 in vivo, including CD8 lymphocytes, natural killer cells, and reticulodendritic cells. Each potentially contributes to the reservoir of infected cells that resist antiviral treatment and to the impairment of immune responses in AIDS. By quantitative PCR for HIV proviral sequences we have now obtained evidence for substantial infection of gammadelta lymphocytes, contributing 3-45% of the proviral load in peripheral blood. A large proportion of gammadelta lymphocytes constitutively expressed the chemokine receptors CCR5 and CXCR4, with evidence for marked up-regulation of CD8 in samples from HIV-infected individuals, corresponding to an activated phenotype. That gammadelta lymphocytes might be susceptible to HIV infection was investigated using in vitro infectivity assays of recombinant HIV-expressing green fluorescent protein, followed by flow cytometry. gammadelta, CD4, and CD8 lymphocytes were each productively infected, with gammadelta lymphocytes showing the greatest susceptibility. For each cell type, blocking assays with an anti-CD4 monoclonal antibody indicated that entry was CD4-dependent.

Citation information in Europe Pubmed Central

Infection with hepatitis B virus (HBV) has been detected in human populations throughout the world, as well as in a number of ape species (Pan troglodytes, Gorilla gorilla, gibbons [Nomascus and Hylobates species] and Pongo pygmaeus). To investigate the distribution of naturally occurring HBV infection in these species and other African Old World monkey species (Cercopithecidae), we screened 137 plasma samples from mainly wild caught animals by polymerase chain reaction (PCR) using several of highly conserved primers from the HB surface (HBs) gene, and for HBs antigen (HBsAg) by ELISA. None of the 93 Cercopithecidae screened (6 species) showed PCR or serology evidence for HBV infection; in contrast 2 from 8 chimpanzees and 5 from 22 gibbons were PCR-positive with each set of primers. Complete genome sequences from each of the positive apes were obtained and compared with all previously published complete and surface gene sequences. This extended phylogenetic analysis indicated that HBV variants from orangutans were interspersed by with HBV variants from southerly distributed gibbon species (H. agilis and H. moloch) occupying overlapping or adjacent habitat ranges with orangutans; in contrast, HBV variants from gibbon species in mainland Asia were phylogenetically distinct. A geographical rather than (sub)species association of HBV would account for the distribution of HBV variants in different subspecies of chimpanzees in Africa, and explain the inlier position of the previously described lowland gorilla sequence in the chimpanzee clade. These new findings have a number of implication for understanding the origins and epidemiology of HBV infection in non-human primates.

It was once thought that the earliest recording of the human voice was made by Thomas Edison in 1877, when he recorded Clearance Buy Karl Lagerfeld K/Kuilted shoulder bag Order Cheap Price Cheap Sale Best Wholesale ls5eTf

Mary had a Little Lamb

BUT, a new recording has turned up. It was made on April 9, 1860 and it is of a woman singing the French folk song “Claire de la lune”. It has now been heard for the first time in 150 years.

You can hear the recording

The interesting thing is that the recording was never intended to be played back – or at least the technology to play it back was never imagined at the time.

It was recorded by a “phonautograph”, which etches waveforms onto paper. French inventor Edouard-Leon Scott de Martinville built the device, which uses a diaphragm that responds to sound to etch the lines onto paper via the soot from an oil lamp.

Scientists have managed to play back the recording using a virtual “stylus”, which is really a computer program that reads the recording from a scanned image. The process sort of reminds me of extremely interesting technique.

A group of audio historians called “ First Sounds ” have been working as archiving early recordings and worked with Paresian Mr. Giovanni, who retreived the sounds from the paper at the Lawrence Berkeley National Laboratory (LBNL) in California.

As the phonautograph was a manually-cranked device (no, they didn’t have crystal timing then!), the speed varied throughout the recording and likewise the pitch changes somewhat over time, giving it a bit of a warbly sound.

The oldest sound ever recorded (also on the phonautograph) is

The oldest recognizable sound ever recorded was that of a tuning fork at 435Hz on the phonautograph. Presented

If you’re into vintage recordings and the history of it all, is an amazing DVD – definitely check it out.

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